When testing for microbial contamination in fuel one of the most important characteristics to understand is the amount of total, active biomass. No test is as fast, easy and accurate as measuring Adenosine Triphosphate (ATP).
LuminUltra Quench–Gone technology is based on the measurement of ATP. Quench–Gone Organic Modified (QGO–M) is designed for organic–based samples, such as fuels and metalworking emulsions. ATP is measured using the firefly luciferase assay, where a sample containing ATP is introduced to a solution containing the enzyme luciferase, which naturally occurs in the tails of fire flies, to produce light. The light is detected in a Luminometer.
QGO–M includes special provisions to separate organics prior to analysis, allowing the measurement of total microbiological concentration with a wide detection range and no assay interference. QGO–M measures the total quantity of microbiological activity through a single analysis within minutes. Compared to other methods of measuring total microbial load (also known as total flora), QGO–M provides immediate feedback, is not prone to interferences, and measures the total living population.
The tests measure the number of picograms of cellular ATP (cATP) per millilitre which can be interpreted as follows:
<10pg/ml cATP = GOOD
10 - 100 pg/ml cATP = PREVENTATIVE ACTION REQUIRED
>100 pg/ml cATP = REMEDIAL ACTION REQUIRED
Use the real–time knowledge gained through QGO–M analysis to assess microbiological contamination; prevent fuel deterioration and optimise maintenance programs. When anti-microbial measures have been taken, such as fuel PureFuel Recirculation & Recovery (R&R) or the installation of a PureFuel Conditioner, the effectiveness can be confirmed with
ATP results.
To begin ATP testing, you will need:
1. Luminometer
2. Luminometer Tubes (for 100 tests)
3. QGOM Test Kit (for 100 tests)
4. QGOMTOOL (for 100 tests)
5. QGOMLAB
Storage Temp. |
Shelf Life |
Quantity |
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| QGO-M Test Kit (for 100 tests) | ||||||
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Luminase Luciferase Enzyme Reagent |
4°C | 4 mo | 15ml | |||
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UltraCheck 1 1 ng/ml ATP Standard |
20°C |
12 mo |
5ml |
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LumiClean Filter Wash Reagent |
20°C |
12 mo |
500ml |
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UltraLyse 7 ATP Extraction Reagent |
20°C |
12 mo |
125ml |
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| UltraLute ATP Dilution Buffer |
20°C | 12 mo | 1000ml | |||
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QGOMTOOL (for 100 tests) |
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1000µl Research Pipette Tips |
300 |
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5ml Research Pipette Tips |
300 |
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15ml Culture Tubes with Caps |
100 |
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20ml Syringes with Luer-Lok |
100 |
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60ml Syringes with Luer-Lok |
5 |
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| 25mm QG Syringe Filters | 100 | |||||
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| 12mm x 55mm/65mm Luminometer tubes | 200 | |||||
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QGOMLAB |
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| 1000µl Research Pipette | 1 | |||||
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| 5ml Research Pipette | 1 | |||||
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| Test Tube Rack | 1 | |||||
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LuminUltra QGO-M
