Petroleum diesels typically hold 60ppm of suspended water. At this level, the water is almost insignificant. Provided the fuel is regularly used and replenished the aforementioned microbial contamination problems do not occur.
THAT WAS THEN, THIS IS NOW
Bio-diesels typically hold up to 25 times more suspended water than petroleum diesel. The worldwide introduction of bio-diesel in recent years, in pursuit of conserving fossil fuel, carbon neutral targets and energy security, has brought a mariner’s fuel problem on land: bio-diesel is a perfect habitat for vigorous microbial growth.
The Lloyds Register published a technical paper (Paper Nº4, Session 1994-95) highlighting critical levels of bacteria in fuel. Less than 500cfu (colony-forming units) per litre is acceptable. At the 500-1000cfu level, Lloyds warns “microbial proliferation occurring” and at over 1000cfu “microbial proliferation; operational problems; investigate thoroughly; use anti-microbial treatment”.
In petroleum diesel the critical levels beyond 500cfu would rarely be seen outside of marine or long term storage situations. Bio-diesel is a different matter – the higher water content provides the microbes with a very attractive environment.