Petroleum diesels typically hold 60ppm of suspended water. At this level, the water is almost insignificant. Provided the fuel is regularly used and replenished the aforementioned microbial contamination problems do not occur.
THAT WAS THEN, THIS IS NOW
Bio-diesels typically hold up to 25 times more suspended water than petroleum diesel. The worldwide introduction of bio-diesel in recent years, in pursuit of conserving fossil fuel, carbon neutral targets and energy security, has brought a mariner’s fuel problem on land: bio-diesel is a perfect habitat for vigorous
microbial growth.
The Lloyds Register published a technical paper (Paper Nº4, Session 1994-95) highlighting critical levels of bacteria in fuel. Less than 500cfu (colony-forming units) per litre is acceptable. At the 500-1000cfu level, Lloyds warns “microbial proliferation occurring” and at over 1000cfu “microbial proliferation; operational problems; investigate thoroughly; use
anti-microbial treatment”.
In petroleum diesel the critical levels beyond 500cfu would rarely be seen outside of marine or long term storage situations. Bio-diesel is a different matter – the higher water content provides the microbes with a very attractive environment.

Consequently the amount of exposure to condensation, leaks or time in undisturbed storage before proliferation begins, is dramatically reduced. Today’s biofuels deteriorate much faster than their petroleum forebears.
Colony Forming Units of bacteria grow on the water-fuel interface becoming heavier until they drop out of the fuel to the bottom of the tank where bio-films and sludge quickly form.
The bacteria feeds on the fuel – but the microbes do not eat ‘all’ of the fuel: they break down the carbon chains which reduces the combustible properties. This leads to:
• poor starting (or failure to start at all)
• excessive smoke
• reduced power output
MICROBIAL CONTAMINATION IS NOT LIMITED TO DIESEL
Aviation jet fuels are subject to the same problems which are the reason for the aviation industry’s rigorous fuel management regimes. Gasoline - petrol - has, until the introduction of bio-content, been the only fuel unaffected by microbial contamination but petrol containing bio-ethanol suffers too.
Universal use of biofuels is at least as profound a change to fuels as the switch to unleaded petrol in the 1990s. Fuel quality management is no longer something that rests only with refineries, ship owners and airlines: microbial contamination affects everyone.